Instrument: INTEGRA ASSIST PLUS
Software: VIALAB v3.7.0 or v3.8.0
Important! This article contains user guides for the semi-automated Parse Biosciences Evercode™ TCR v3 and TCR Mega v3 workflow on the INTEGRA ASSIST PLUS robotic system. The protocol replaces "Section 1. In Situ Cell Barcoding", "Section 2. cDNA Purification and Amplification", and "Section 3. Sequencing Library Preparation" of the standard Evercode TCR v3 User Guide. Section 4 of the user guide will need to be performed manually.
Non-automated workflows for Evercode TCR v3 can be found here: Evercode TCR v3 User Guides.
At the bottom of this article, please find the following attached User Guides:
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Evercode TCR v3 with INTEGRA ASSIST PLUS (Sections 1-3) v1.4
- For use with: ECIT2300 (Human), ECIT1310 (Mouse)
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Evercode TCR Mega v3 with INTEGRA ASSIST PLUS (Sections 1-3) v1.6
- For use with: ECIT2500 (Human), ECIT1510 (Mouse)