Fixed samples can be stored at -80°C for up to 6 months. Storing samples directly in the freezer without controlled cooling may lead to damage and compromise data quality. Therefore, samples should always be cooled slowly.
Parse Biosciences offers tube-based and plate-based fixation workflows. If the fixed samples are stored in a 1.5 mL tube, a Mr. Frosty™ Freezing Container (or equivalent device) should be used to cool the sample at -1°C/minute for long term storage at -80°C. If the fixed samples are stored in a PCR plate or 0.2mL strip tubes, the samples should be placed in a room temperature styrofoam cooler with a closed lid and stored at -80°C to slowly cool the samples.
Note: If the fixed samples are frozen in a plate, it is important to seal the plate with a seal that can withstand storage at -80°C.