Samples fixed with EvercodeTM Cell Fixation or Evercode Nuclei Fixation can be stored at -80°C for up to 6 months. Samples fixed with Evercode Low Input Cell Fixation or Evercode Low Input Nuclei Fixation can be stored at -80°C for up to 4 months.
Storing samples directly in the freezer without controlled cooling may lead to damage and compromised data quality. Therefore, samples should always be cooled slowly with the following technique. If the fixed samples are stored in a 1.5 mL tube, a Mr. Frosty™ Freezing Container (or equivalent device) should be used to cool the sample at -1°C/minute for long term storage at -80°C. If the fixed samples are stored in a PCR plate or 0.2mL strip tubes, the samples should be placed in a room temperature styrofoam cooler with a closed lid and stored at -80°C to slowly cool the samples.
Parse Biosciences also offers tube-based and plate-based fixation workflows for high throughput processing of samples. Samples prepared in this way should be frozen and stored with the same considerations as outlined above.
Note: If the fixed samples are frozen in a plate, it is important to seal the plate with a seal that can withstand storage at -80°C. Please refer to the Evercode Fixation user guides for more information.