CRISPR Detect is compatible with CROP-seq methods that generate a polyadenylated transcript expressed downstream of a human U6 promoter. The table below includes some examples, but it is not exhaustive. Please also see the CRISPR Detect Vector Compatibility Guide for more information.
Vector | Selection | Other Features | Addgene Number | Pubmed ID |
CROPseq-Guide-Puro | Puromycin | 86708 | 28099430 | |
CROPseq-puro-v2 | Puromycin | Modified sgRNA scaffold for increased Cas9 efficiency | 127458 | 31626775 |
CROPseq-Guide-Zeo | Zeocin | 127173 | 31626775 | |
CROP-seq-opti | Puromycin | CRISPRi optimized backbone | 106280 | 29457792 |
CROP-sgRNA-MS2 | Puromycin or mCherry | sgRNA scaffold sequence contains two MS2 | 153457 | 32634384 |
CROPseq-Guide-EFS-SpCas9-P2A-EGFP | EGFP | EF-1α replaced with EFS promoter | 99248 | |
EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212) | Neomycin or EGFP | Cas12a scaffold, enabling dual guide screening | 149722 | 32661245 |
Crop-Seq-BFP-WPRE-TS-hU6-BsmbI | BFP | Lineage tracing vector system | 137993 | 35094325 |