Unique dual indices (UDIs) minimize the impact of index hopping that can potentially occur during Illumina sequencing and results in the misassignment of reads to a different Illumina index.
We offer two sets of Unique Dual Indices for our Evercode TCR, CRISPR Detect, and Evercode WT kits:
UDI Plate - WT (PN: UDI1001) is used to prepare Evercode Whole Transcriptome sequencing libraries. Evercode Whole Transcriptome libraries contain 5’ and 3’ TruSeq adapters, and the UDI Plate - WT adds TruSeq read 1 and Truseq read 2.
UDI Plate - EC (PN: UDI1002) is used to prepare TCR and CRISPR sequencing libraries. These libraries contain 5’ Nextera and 3’ Truseq adapters, and the UDI Plate - EC adds Nextera read 1 and Truseq read 2.
One UDI well is used for each sublibrary. UDI plates are compatible with all kit sizes and contain 48 single use wells, each with a different UDI. For more information please contact email@example.com.