We recommend 2,000 reads/cell for Gene Capture sequencing libraries prepared using the Immune1000 Panel. For Gene Capture libraries prepared using a custom panel, we also recommend using 2,000 reads per cell as a starting point, however the exact depth required will depend on the size of the panel.
For sequencing, refer to run configuration details in the appropriate user manual used to generate the Evercode Whole Transcriptome Libraries that were input to the Gene Capture workflow.
For WT sequencing requirements see: What are the run configuration and sequencing requirements for WT libraries?
For UDI sequences see: Unique Dual Indices (UDI) Sequences
For single index sequences see: Single Index Sequences